Lamin A

Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. \nThe nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organization with the other intermediate filament proteins in that they are fibrous molecules that have an amino terminal globular head, a central rod of a-helices and a carboxy terminal globular domain. \nMany biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. \nBesides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organized into loops, which are attached to the nuclear matrix. This organization is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.\n\nApplications:\nSuitable for use in Immunocytochemistry, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: Frozen sections\nFlow Cytometry: 1:100-1:200\nImmunohistochemistry: 1:100-1:200; Frozen sections, Use ABC detection system\nWestern Blot: 1:100-1:1000\nOptimum dilutions to be determined by researcher.\n\nHybridoma:\nHybridization of P3/X63.Ag8.653 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1220-03

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.

P Type

Mab

Purity

Purified

Isotype

IgG3,k

References

1. Hozak, P., et al., J. Cell Sci. 108: 635-644 (1995). 2. Machiels, B.M., et al., Eur. J. Cell Biol. 67: 328-335 (1995). 3. Broers, J.L., et al., Histochem. Cell Biol. 107: 505-517 (1997). 4. Pugh, G.E., et al., J. Cell Sci. 110: 2483-2493 (1997). 5. Machiels, B.M., et al., J. Pathol. 182: 197-204 (1997). 6. Jansen, M.P., et al., Histopathology 31: 304-312 (1997). 7. Neri, L.M., et al., J. Cell Biochem. 75: 36-45 (1999). 8. Neri, L.M., et al., J. Cell Physiol. 178: 284-295 (1999). 9. Broers, J.L., et al., J. Cell Sci. 112: 3463-3475 (1999). 10. Broers, J.L., et al., Eur. J. Cell Biol. 81: 677-691 (2002). 11. De Sandre-Giovannoli, A., et al., Science 300: 2055 (2003). 12. Eriksson, M., et al., Nature 423: 293-298 (2003).

Additional Info

Reacts with lamin A exclusively; epitope located between aa598-611. Species Crossreactivity: Human, mouse, rat, bovine, and canine.