Laminin BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Laminin in cell culture supernates, serum and plasma (heparin, EDTA).\n\nLaminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation.2 The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids.1 The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.4 The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.5 The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.6 The standard product used in this kit is isolated from plasma.\n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nB0YJ33\n\nEntrez Gene ID:\n3909\n\nKit Components:\n96-well plate pre-coated with anti-Laminin, Human\nLyophilized recombinant Laminin, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143945

Size

96Tests

References

1. Haaparanta, T.; Uitto, J.; Ruoslahti, E.; Engvall, E. Molecular cloning of the cDNA encoding human laminin A chain. Matrix 11: 151-160, 1991.\n2. Sasaki, M.; Kato, S.; Kohno, K.; Martin, G. R.; Yamada, Y. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc. Nat. Acad. Sci. 84: 935-939, 1987. \n3. Nagayoshi, T.; Mattei, M.-G.; Passage, E.; Knowlton, R.; Chu, M.-L.; Uitto, J. Human laminin A chain (LAMA) gene: chromosomal mapping to locus 18p11.3. Genomics 5: 932-935, 1989. \n4. Jaye, M.; Modi, W. S.; Ricca, G. A.; Mudd, R.; Chiu, I.-M.; O'Brien, S. J.; Drohan, W. N. Isolation of a cDNA clone for the human laminin-B1 chain and its gene localization. Am. J. Hum. Genet. 41: 605-615, 1987. \n5. Sasaki, M.; Yamada, Y. The laminin B2 chain has a multidomain structure homologous to the B1 chain. J. Biol. Chem. 262: 17111-17117, 1987. \n6. Mattei, M.-G.; Weil, D.; Pribula-Conway, D.; Bernard, M. P.; Passage, E.; Van Cong, N.; Timpl, R.; Chu, M.-L. cDNA cloning, expression and mapping of human laminin B2 gene to chromosome 1q31. Hum. Genet. 79: 235-241, 1988. \n