LANA 2 (Latency-Associated Nuclear Antigen 2, LANA-2)

LANA2 (latency-associated nuclear antigen) is an inhibitor of p53-induced transcription in reporter assays and may be an apoptosis antagonist. It is a nuclear protein associated with Kaposi's sarcoma-associated herpes virus (KSHV), or human herpes virus 8. It is found in abundance in the nuclei of cultured cells from KSHV-infected hematopoietic tissues, such as PEL (primary effusion lymphoma) and CD (multicentric Castleman's disease). It does not appear to be expressed in KS (Kaposi's sarcoma) lesions.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen and paraffin): 1:10-1:2000\nELISA: 1:100-1:2000\nImmunofluorescence (IC): 1:10-1:2000\nWestern Blot: 2-4ug/ml detects a band at ~66-70kD.\nImmunoprecipitation: 1:10-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1237-20

Size

100ul

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Marcos-Villar L, Lopitz-Otsoa F, Gallego P, et al. Kaposi's Sarcoma-Associated Herpesvirus Protein LANA2 Disrupts PML Oncogenic Domains Inhibits PML-Mediated Transcriptional Repression of the Survivin Gene. J Virol 2009;83(17):8849-8858. [pubmed: 19553342] 2. Shin, YC, et al. Kaposi's sarcoma-associated herpesvirus viral IFN regulatory factor 3 stabilizes hypoxia-inducible factor-1 alpha to induce vascular endothelial growth factor expression. Cancer Res. 2008 Mar 15;68(6):1751-9.

Additional Info

Recognizes aa143-310 from KSHV LANA2 in KSHV infected B cells. Species Crossreactivity: human and mouse viral KSHV proteins.