Lauryl Sulfate Broth w/MUG (Powder)

Rapid detection of E. coli from water and food samples by fluorogenic detection methods.\n\nLauryl Sulfate Broth was formulated by Mallmann and Darby for detection of coliform bacteria in water. This is a selective medium because it contains sodium lauryl sulfate which inhibits Gram positive microorganisms. Lactose is added to detect rapid lactose fermentation by coliforms. The addition of 4- methylumbelliferyl-B-D-glucuronide (MUG) to Lauryl Sulfate Broth allows for the rapid detection (4-24 hours) of E. coli-When the incubated medium is observed for fluorescence using a long-wave UV light (365 nm) source. It can also detect anaerogenic E-coli strains. Most strains of E. coli produce B- glucuronidase which hydrolyzes MUG to the fluorogenic compound 4- methyl-umbelliferone. Studies by Feng and Hartman noted that most E coli strains (96%) and all enterotoxigenic E coli strains (100%) and a moderate number of Salmonella (17%) and Shigella (40%) stains produce B-glucuronidase. False positives can also be caused by Streptococcus sp.\n\nQuality Control Summary:\nExpected cultural response after 18-24 hours at 35.0 degrees C.\n \nOrganism Result\nEscherichia coli ATCC 25922 Growth, Gas +, Fluorescence + \nProteus mirabilis ATCC 12453 Growth, Gas -, Fluorescence - \nStaphylococcus aureus ATCC 25923 No Growth\n \n\n\n

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SPECIFICATIONS

Catalog Number

L1400-05

Size

500g

References

1. Mallmann, W. L., and C. W. Darby. 1941. Uses of a lauryl sulphate tryptose broth for the detection of coliform organsisms. Am J. Public Health. 31:127.