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Leptin (Ob, Obese Protein, Obesity Factor)

Cat no: L1669-03A

Leptin (Ob, Obese Protein, Obesity Factor)

Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, ddH2O, 0.09% sodium azide. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1669-03A

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O, 0.09% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Paracchini, V. et al. (2005) Genetics of leptin and obesity: a HuGE review. Am. J. Epidemiol. 162: 101-114.

Additional Info

Recognizes human leptin, at 16kD.

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