Leptin (Ob, Obese Protein, Obesity Factor)

Leptin, the product of the ob (obese) gene, is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence the reproductive function.\n\nApplications: \nSuitable for use in ELISA, Western blotting.\n\nTiter: Defined by indirect ELISA is >1:100,000 for antibody concentration 1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.\n\nReconstitution: Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1670-21A

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Sterile filtered and lyophilized from 1mg/ml 0.05M phosphate buffer, 0.1M sodium chloride, pH 7.2.

P Type

Pab

Purity

Immunoaffinity chromatography on a column with immobilized human recombinant rat leptin.

References

1. Perez C, Fernandez-Galaz C, Fernandez-Agullo T, Arribas C, Andres A, Ros M, Carrascosa JM. : Leptin Impairs Insulin Signaling in Rat Adipocytes. Diabetes. 2004 Feb;53(2):347-353.\n\n2. Polidori C, Luciani F, Fedeli A, Geary N, Massi M.: Leptin fails to reduce ethanol intake in Marchigian Sardinian alcohol-preferring rats. Peptides. 2003 Sep;24(9):1441-4.\n\n3. Giovambattista A, Suescun MO, Nessralla CC, Franca LR, Spinedi E, Calandra RS.: Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats. Neuroendocrinology. 2003 Nov;78(5):270-9.\n\n4. Cassis LA, English VL, Bharadwaj K, Boustany CM. : Differential effects of local versus systemic angiotensin II in the regulation of leptin release from adipocytes. Endocrinology. 2004 Jan;145(1):169-74. Epub 2003 Sep 25.\n\n5. Birk RZ, Regan KS, Brannon PM. : Circulating leptin levels in newborn rats: a significant postnatal developmental effect, independent of dietary polyunsaturated fat levels. Life Sci. 2003 Oct 10;73(21):2761-7.\n\n6. Kutlu S, Canpolat S, Sandal S, Ozcan M, Sarsilmaz M, Kelestimur H. : Effects of central and peripheral administration of leptin on pain threshold in rats and mice. Neuroendocrinol Lett. 2003 Jun-Aug;24(3-4):193-6.

Additional Info

The recombinant rat leptin is 100% homologous with the rat serum leptin.