Leukotriene B4 EIA Kit

LTB4 is synthesized from arachidonic acid by the combined action of 5-LO and LTA4 hydrolase. LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10-10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs. At higher concentrations, (1.0 x 10-7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production. Plasma levels of LTB4 increase from <100 pg/ml to >100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 and is not excreted in the urine.

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SPECIFICATIONS

Catalog Number

520111

Form

96 Well

P Type

Assay Kits|Leukotrienes|Eicosanoids

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Leukotriene B4 (LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and LTA4 hydrolase. LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10^-10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs. At higher concentrations, (1.0 x 10^-7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production. Plasma levels of LTB4 increase from <100 pg/ml to >100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by .beta.-oxidation at the .omega.-end to .omega.-carboxy dinor LTB4 and .omega.-carboxy tetranor LTB3. LTB4 is not excreted in the urine.