Lims1, Control Peptide

Synthetic peptide AGSNENDDQNLGAKRR (linear) corresponding to aa166-181 of Lims1. Control peptide for L2000-01.\n\nThe expression of Lim1 is very similar to that of the PD gene aristaless. al is expressed in the most distal part of the leg discs, and in two rings in the peripheral and medial regions. Confocal immunofluorescence staining has shown that the expression of Lim1 and al are coincident in the most distal parts of the leg and antenna disc. Furthermore, there are similarities between the al and Lim1 mutant phenotypes, since in al mutants the claw organ, the sternopleural bristles in the leg and the arista are missing or reduced. A possible functional relationship between the two genes was explored. Since al is expressed earlier than Lim1, one possibility is that Lim1 expression is regulated by al. The expression of Lim1 was examined in al mutants, and Lim1 was found to be lost in the presumptive tip of the leg where both genes are co-expressed. In contrast, al expression is normal in Lim1 mutants. These results suggest that the Lim1 locus is regulated by the al gene, one possibility being that the Lim1 gene is a direct downstream target of the homeodomain Al protein (Pueyo, 2000).\n\nStorage and Stability: \nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n \n

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SPECIFICATIONS

Catalog Number

L2200-01A

Size

1mg

Form

Supplied as a lyophilized powder.

References

Agulnick, A. D., et al. (1996). Interactions of the LIM-domain-binding factor Lbd1 with LIM homeodomain proteins. Nature 384: 270-272. Medline abstract: 97078753 \n\nBeck, C. W. and Slack, J. M. W. (1998). Analysis of the developing Xenopus tail bud reveals separate phases of gene expression during determination and growth. Mech. Dev. 72(1-2): 41-52. Medline abstract: 98202511 \n\nBreen, J. J., et al. (1998). Interactions between LIM Domains and the LIM Domain-binding Protein Ldb1. J. Biol. Chem. 273(8): 4712-4717. Medline abstract: 98136177 \n\nChan, T.-c., Takahashi, S. and Asashima, M. (2000). A Role for Xlim-1 in pronephros development in Xenopus laevis. Dev. Biol. 228: 256-269. Medline abstract: 20564079 \n\nDawid, I. B., Toyama, R. and Taira, M. (1995). LIM domain proteins. C R Acad Sci III 318: 295-306. Medline abstract: 95308295 \n\nEnsini, M., et al. (1998). The control of rostrocaudal pattern in the developing spinal cord: specification of motor neuron subtype identity is initiated by signals from paraxial mesoderm. Development 125: 969-982. Medline abstract: 98130557 \n\nFernandez-Funez, P., et al. (1998). The relative expression amounts of apterous and its co-factor dLdb/Chip are critical for dorso-ventral compartmentalization in the Drosophila wing. EMBO J. 17: 6846-6853. Medline abstract: 99059722 \n\nFujii, T., et al. (1994). Expression patterns of the murine LIM class homeobox gene lim1 in the developing brain and excretory system. Dev. Dyn. 199(1): 73-83. Medline abstract: 94220754 \n\nGupta, B. P. and Sternberg, P. W. (2002). Tissue-specific regulation of the LIM homeobox gene lin-11 during development of the Caenorhabditis elegans egg-laying system. Dev. Bio. 247: 102-115. Medline abstract: 12074555 \n\nGupta, B. P., Wang, M. and Sternberg, P. W. (2003). The C. elegans LIM homeobox gene lin-11 specifies multiple cell fates during vulval development. Development 130: 2589-2601. Medline abstract: 12736204 \n\nHobert, O., et al. (1998). Control of neural development and function in a thermoregulatory network by the LIM homeobox gene lin-11. J. Neurosci. 18(6): 2084-2096. Medline abstract: 98151395 \n\nHukriede, N. A., et al. (2003). Conserved requirement of Lim1 function for cell movements during gastrulation. Dev. Cell 4: 83-94. Medline abstract: 12530965 \n\nKaravanov, A. A., et al. (1996). The LIM homeodomain protein Lim-1 is widely expressed in neural, neural crest and mesoderm derivatives in vertebrate development. Int. J. Dev. Biol. 40(2): 453-61. Medline abstract: 96385785 \n\nKobayashi, A., Shawlot, W., Kania, A. and Behringer, R. R. (2003). Requirement of Lim1 for female reproductive tract development. Development 131: 539-549. Medline abstract: 14695376 \n\nLi, Y., et al. (1999). Sequence and genomic organization of the mouse Lim1 gene. Mamm. Genome 10(5): 444-6. Medline abstract: 99269909 \n\nLilly, B., et al. (1999). The LIM homeodomain protein dLim1 defines a subclass of neurons within the embryonic ventral nerve cord of Drosophila. Mech. Dev. 88(2): 195-205. Medline abstract: 20005796 \n\nMorcillo, P., et al. (1997). Chip, a widely expressed chromosomal protein required for segmentation and activity of a remote wing margin enhancer in Drosophila. Genes Dev. 11(20): 2729-2740. Medline abstract: 97477378 \n\nO'Keefe, D. D., Thor, S. and Thomas, J. B. (1998). Function and specificity of LIM domains in Drosophila nervous system and wing development. Development 125(19): 3915-3923. Medline abstract: 98399917 \n\nPerea-Gomez, A., et al. (1999). HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo. Development 126: 4499-4511. Medline abstract: 99429810 \n\nPueyo, J. I., et al. (2000). Proximal-distal leg development in Drosophila requires the apterous gene and the Lim1 homologue dlim1 Development 127: 5391-5402. Medline abstract: 20530492 \n\nRebbert, M. L. and Dawid, I. B. (1997). Transcriptional regulation of the xlim-1 gene by activin is mediated by an element in intron I. Proc. Natl. Acad. Sci. 94(18): 9717-9722. Medline abstract: 97420748 \n\nSarafi-Reinach, T. R., et al. (2001). The lin-11 LIM homeobox gene specifies olfactory and chemosensory neuron fates in C. elegans. Development 128: 3269-3281. Medline abstract: 11546744 \n\nShawlot, W. and Behringer, R. R. (1995). Requirement for Lim1 in head-organizer function. Nature 374(6521): 425-30. Medline abstract: 95214746 \n\nShawlot, W., et al. (1999). Lim1 is required in both primitive streak-derived tissues and visceral endoderm for head formation in the mouse. Development 126: 4925-4932. Medline abstract: 20002425 \n\nSockanathan, S. and Jessell, T. M. (1998). Motor neuron-derived retinoid signaling specifies the subtype identity of spinal motor neurons. Cell 94(4): 503-14. Medline abstract: 98394476 \n\nTaira, M., Jamrich, M., Good, P. J. and Dawid, I. B. (1992). The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos. Genes Dev. 6(3): 356-66. Medline abstract: 92192449 \n\nTaira, M., Otani, H., Jamrich, M. and Dawid, I. B. (1994a). Expression of the LIM class homeobox gene Xlim-1 in pronephros and CNS cell lineages of Xenopus embryos is affected by retinoic acid and exogastrulation. Development 120(6): 1525-36. Medline abstract: 94326650 \n\nTaira, M., et al. (1994b). Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus. Nature 372(6507): 677-9. Medline abstract: 95082944 \n\nTaira, M., Saint-Jeannet, J.-P. and Dawid, I. B. (1997). Role of the Xlim-1 and Xbra genes in anteroposterior patterning of neural tissue by the head and trunk organizer. Proc. Natl. Acad. Sci. 94: 895-900. Medline abstract: 97175698 \n\nThor, S., Andersson, S. G., Tomlinson, A., Thomas, J. B., 1999. A LIM-homeodomain combinatorial code for motor-neuron pathway selection. Nature 397: 76-80. Medline abstract: 99107375 \n\nToyama, R., et al. (1995). Nodal induces ectopic goosecoid and lim1 expression and axis duplication in zebrafish. Development 121: 383-391. Medline abstract: 95285760 \n\nTsuchida, T., et al. (1994). Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes. Cell 79: 957-970. Medline abstract: 95094281 \n\nTsuji, T., et al. (2000). Requirements of Lim1, a Drosophila LIM-homeobox gene, for normal leg and antennal development. Development 127: 4315-4323. Medline abstract: 20461140 \nLim1: Biological Overview | Evolutionary Homologs | Regulation | Developmental Biology | Effects of Mutation \n\nflybase-help AT morgan.harvard.edu