Lipid droplets are a fundamental component of intracellular lipid homeostasis in all cell types, and they can provide a rapidly mobilized lipid source for many important biological processes such as membrane trafficking and cell signaling. Cayman's Lipid Droplets Fluorescence Assay can be used to study regulators of lipid droplet biogenesis in a variety of conditions, including inflammation. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets. This kit provides sufficient reagent to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format.