Lipopolysaccharide Binding Protein (LBP, LPSBP)

LPS binding protein (LBP) is an approximately 60kD acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.\n\nApplications:\nSuitable for use in Functional Studies in vitro and in vivo, Western Blot and Immunoassays. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10\nOptimal dilutions to be determined by the researcher.\nBefore use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L2525-25A

Size

100ug

Applications

WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1.Le Roy, D et al; Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP complexes to CD14. J Immunol 1999, 162: 7454 2. Le Roy, D et al; Critical role of lipopolysaccharide-binding protein and CD14 in immune response against gramnegative bacteria. J Immunol 2001, 169: 2759

Additional Info

Recognizes mouse natural and recombinant LBP. The antibody isa type I antibody blocking the LPS binding to LBP.

Alternative Names

LBP