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Lotus tetragonolobus Lectin (LTL) (Biotin)

Cat no: L3445-10B

Lotus tetragonolobus Lectin (LTL) (Biotin)

Lotus tetragonolobus lectin is a family of closely related glycoproteins having 2 or 4 subunits of about 28,000D. The isoelectric points differ somewhat but these isolectins probably have similar specificities toward alpha-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are markedly different between these fucose-specific lectins. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nInhibiting/Eluting Sugar: \n50mM-100mM L-fucose\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L3445-10B

Size

2mg

Applications

ELISA, IHC, WB

Conjugates

Biotin

Form

Supplied as a powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca++. Reconstitute with 1ml dH2O. Labeled with Biotin.

Alternative Names

Asparagus Pea, Winged Pea

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