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LRP, MVP (Lung-resistance Related Protein, Major Vault Protein)

Cat no: L4500-06

LRP, MVP (Lung-resistance Related Protein, Major Vault Protein)

Major vault protein (MVP) is identical to lung resistance related protein (LRP). Its shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocortoid receptors are also able to co-immunoprecipitate the MVP. Reportedly, the lung resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplasitc tissues and cells lines indicating a poor response to chemotherapy.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunoprecipitation (Native verified): 2ug/mg protein lysate Use Protein G.\nWestern Blot: 1-2ug/ml for 2 hrs at RT.\nImmunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Controls:\nSKBR3 cells and breast carcinomas\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L4500-06

Size

500ul

Applications

FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See L4500-06X.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Abbondanza, C., et al., (1998) J. Cell. Biol. 141(6):1301-1310.

Additional Info

Recognizes human major vault protein (MVP) at 104-110kD. \nSpecies Crossreactivity: rat.

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