TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes incuding cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells. Recombinant human TNF-beta is a secreted 172aa protein,18.6kD, which forms an active non-disulfide linked homotrimer structure in solution. Due to glycosylation, TNF-beta has an approximate MW of 19.3kD based on SDS-PAGE gel and Mass Spectrometry.
Source:
Recombinant protein corresponding to human TNF-beta, expressed in (BTI-Tn-5B1-4) High-5 Insect Cells.
Biological Activity:
Determined by the cytolysis of murine L929 cells in the presence of Actinomycin D. The ED50 was found to be 0.05ng/ml.
Specific Activity:
2x10e7units/mg
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
GVGLTPSAAQ TARQHPKMHL AHSTLKPAAH LIGDPSKQNS LLWRANTDRA FLQDGFSLSN NSLLVPTSGI YFVYSQVVFS GKAYSPKATS SPLYLAHEVQ LFSSQYPFHV PLLSSQKMVY PGLQEPWLHS MYHGAAFQLT QGDQLSTHTD GIPHLVLSPS TVFFGAFAL
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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