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Macrophage Colony Stimulating Factor, Recombinant, Human (CSF1)

Cat no: M1201-03C

Macrophage Colony Stimulating Factor, Recombinant, Human (CSF1)

Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts.\n\nRecombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. .\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nEndotoxin: (same/less than)1EU/1ug (LAL)\n\nAmino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD\nNTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN\n\nMolecular Weight: 20.7kD (179aa)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M1201-03C

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 2mM DTT, 10% glycerol.

Purity

~90% by SDS-PAGE, after refolding of the isolated inclusion bodies in a renaturation buffer.

References

Wei S., et al. (2006). J Leukoc Biol. 80(6):1445-53. Woo KM., et al. (2002). Exp Mol Med. 34(5):340-6.\n

Alternative Names

CSF1

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