Macrophage Inflammatory Protein 1 alpha (MIP-1a, MIP-1 alpha, CCL3, Chemokine C-C Motif Ligand 3, G0/G1 Switch Regulatory Protein 19-1, G0S19-1, Heparin Binding Chemotaxis Protein, L2G25B, LD78 alpha, PAT 464.1, SCYA3, SIS alpha, SIS beta, Small Inducible

MIP1 alpha belongs to the chemokine beta family. In vitro MIP1 alpha stimulates H2O2 production in human neutrophils. MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor 1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.\n\nApplications: \nSuitable for use in N Neut, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M1202-03B

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

PBS. pH 7.4

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

Graham GJ et al. Identification and characterization of an inhibitor of haemopoietic stem cell proliferation. Nature 344:442-4 (1990). <PUBMED:2320111>Wolpe SD et al. Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties. J Exp Med 167:570-81 (1988). <PUBMED:3279154>

Additional Info

Anti-Human MIP1 alpha neutralizes the of bioactivity of recombinant, human MIP1 alpha, but not that of recombinant, mouse MIP1 alpha. In indirect ELISA and immunoblotting, this antibody displays approximately 1% crossreactivity with recombinant, mouse MIP1 alpha and recombinant, mouse MIP1 beta. The product shows no crossreactivity with other tested cytokines using indirect ELISA. Species Crossreactivity: Crossreacts with Human and Mouse.