Macrophage Inflammatory Protein 1

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells. \n\nRecombinant human MIP-1 alpha is a 7.8kD protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.\n\nSequence: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA\n\nSource: E. coli \n\nForm: Lyophilized protein with no additives.\n\nStability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0 degrees C.\n\nPurity: (same/more than) 99% by SDS-PAGE and HPLC analysis. \n\nEndotoxin: 0.1 ng/ug\n\nReconstitution: Lyophilized MIP-1alpha is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1mg/ml. This solution can be diluted into other buffered solutions or stored frozen for future use.\n\nBiological Activity: The maximal chemotactic activity of human MIP-1alpha on human monocytes was achieved at 50ng/ml.\n\nMolecular Weight: 7.8kD \n\nResponding Cells (partial list): Monocytes, stem cells\n\nConcentration Range for most in vitro applications: 5.0-50ng/ml

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