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Products Malachite Green Phosphate Assay Kit
| Catalogue number: | 10009325 |
| Weight: | 0 |
| Form: | 1 ea |
| P type: | Assay Kits|Small Molecule Detection (Non-EIA) |
| Shipping temp: | 4 |
| Storage temp: | 4 |
| Additional info: | Cayman's Malachite Green Phosphate Assay Kit provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm. Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates. This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications. The assay is formatted to a 96-well format, but could easily be modified for use in 384-well or cuvette-based assays. |
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