MAP Kinase 10, Active, Recombinant, Human, GST-Tag (Mitogen-activated Protein Kinase 10, MAPK 10, MAPK10, PRKM10, c-Jun N-terminal Kinase 3, JNK3, JNK3A, MAP Kinase p49 3F12, p493F12, p54bSAPK, Stress-activated Protein Kinase 1b, SAPK1b, Stress-activated

MAPK10 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kinase 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. \n\nSource:\nRecombinant corresponding to aa2-422 from human MAPK10, fused to GST-tag at N-terminal, expressed in Sf9 cells via a baculovirus expression system.\n\nMolecular Weight:\n~75kD\n\nApplications:\nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nSpecific Activity:\n~22pmol/min/ug\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137311

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 20% glycerol, 3mM DTT.

Purity

Purified (~55%)

References

1. Lisnock J, et al. (2000). Biochemistry 39(11): 3141-8. 2. Hu MC, et al. (1997). Oncogene 15(19): 2277-87. 3. Ying J, et al. (2006). Leukemia 20(6): 1173-1175.