MAP3K5, CT (Mitogen-activated Protein Kinase Kinase Kinase 5, MAPKKK5, Apoptosis Signal-regulating Kinase 1, ASK1, ASK-1, MAPK/ERK Kinase Kinase 5, MEK Kinase 5, MEKK 5, MEKK5) (APC)

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374aa with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nImmunohistochemistry: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2867-52K-APC

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004). 2.Huang, S., et al., J. Biol. Chem. 279(35):36490-36496 (2004). 3.Yasinska, I.M., et al., Arch. Biochem. Biophys. 428(2):198-203 (2004). 4.Goldman, E.H., et al., J. Biol. Chem. 279(11):10442-10449 (2004). 5.Park, H.S., et al., J. Biol. Chem. 279(9):7584-7590 (2004).

Additional Info

Recognizes human MEKK5.

Alternative Names

EC=2.7.11.25