MAP4K4, phosphorylated (Ser629) (Mitogen-activated Protein Kinase Kinase Kinase Kinase 4, HPK/GCK-like Kinase HGK, HGK, KIAA0687, MAPK/ERK Kinase Kinase Kinase 4, MEK Kinase Kinase 4, MEKKK 4, MEKKK4, Nck-interacting Kinase, NIK) (APC)

M4K4, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.\n\nApplications: \nSuitable for use in ELISA and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M3949-74A-APC

Size

200ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Wright, J.H., et al., Mol. Cell. Biol. 23(6):2068-2082 (2003). 2.Yao, Z., et al., J. Biol. Chem. 274(4):2118-2125 (1999). 3.Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).

Additional Info

Recognizes human MAP4K4 when phosphorylated at Ser629.

Alternative Names

EC=2.7.11.1, FLH21957, FLJ10410, FLJ20373, FLJ90111