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Matrix Metalloproteinase 13 (MMP-13, Collagenase-3)

Cat no: M2429-04

Matrix Metalloproteinase 13 (MMP-13, Collagenase-3)

Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. \n\nApplications:\nSuitable for use in Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen and formalin-fixed/paraffin): 5-10ug/ml for 30-60 minutes at RT. \nStaining of formalin-fixed tissues requires boiling of tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. \nImmunoprecipation: (Native verified) 2ug/mg protein lysate. Use Protein G. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nConditioned, serum-free medium from (TPA-treated) human chondrosarcoma SW1353 cells. Placenta, breast, ovarian carcinomas. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2429-04

Size

100ug

Applications

IHC, IP

Hosts

Mouse

Reactivities

Hum, Mouse, Rabbit

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G chromatography.

Isotype

IgG1

Additional Info

Recognizes human MMP-13 at ~60kD and ~48kD which are identified as pro (latent) and active forms of matrix metalloproteinase-13. Shows no crossreactivity with the pro and active forms of other MMPs. Species Crossreactivity: rabbit and mouse.

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