Maturation Inducing Steroid (MIS; 17.alpha.,20.beta.-dihydroxy-4-pregnen-3-one) is one of the key mediators for oocyte maturation in fish. Several different names are synonymous with this molecule including Maturation Inducing Hormone (MIH), Fish Maturation Hormone (FMH), and 17.alpha.,20.beta.-dihydroxy progesterone (17.alpha.,20.beta.-DiOH-P). MIS is produced in ovarian follicular cells and acts directly on the oocyte via a specific receptor to cause oocyte maturation. Cayman Chemical's MIS EIA tracer, antiserum, and standard are designed for the measurement of MIS in a standard solid-phase enzyme immunoassay format.