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MCL1 (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like Protein 3, Bcl2-L-3, Bcl-2-related Protein EAT/mcl1, mcl1/EAT, BCL2L3)

Cat no: M2749-01J

MCL1 (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like Protein 3, Bcl2-L-3, Bcl-2-related Protein EAT/mcl1, mcl1/EAT, BCL2L3)

Mcl-1 (Myeloid cell leukemia-1) is a member of the bcl-2 family. The carboxy terminal of Mcl-1 and bcl-2 share significant sequence homology. Expression of Mcl-1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Like bcl-2, Mcl-1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:25-1:100 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2749-01J

Size

250ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

Additional Info

Recognizes human MCL1.

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