MCM2, phosphorylated (Ser108) (Mini Chromosome Maintenance Protein 2, MCM2 Minichromosomal Maintenance Deficient 2 Mitotin, BM28, Cyclin-like 1, CCNL1, Cell Devision Cycle-like 1, CDC like-1, CDCL1, cdc19, D3S3194, DNA Replication Licensing Factor MCM2, K

MCM2 is a putative DNA replicative helicase and member of the MCM family, critical during the initiation of DNA replication. During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Studies have shown that the phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and that phosphorylation results in a conformational change of the complex and activation of the helicase activity.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5,000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2749-29B

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in tris-citrate/phosphate, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Forsburg, S.L. (2004) Eukaryotic MCM proteins: Beyond replication initiation. Microbiol. Mol. Biol. Rev. 68: 109-131.\n2. Tsuji, T. et al. (2006) Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol. Biol. Cell 17: 4459-4472.

Additional Info

Recognizes the nuclear protein MCM2 (mini chromosome maintenance protein 2) when phosphorylated at Ser108.