MDM2, Active, Recombinant, Human, c-Myc GST-Tag (Ubiquitin-protein Ligase E3 Mdm2, p53-binding Protein Mdm2, Oncoprotein Mdm2, Double Minute 2 Protein, Hdm2)

MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. The suppression of p53 activity by an amplified Mdm2 gene has been observed in many tumours, including soft tissue sarcomas, osteosarcomas and breast tumours.MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. The suppression of p53 activity by an amplified Mdm2 gene has been observed in many tumours, including soft tissue sarcomas, osteosarcomas and breast tumours.\n\nSource: \nRecombinant corresponding to full length human MDM2, fused to GST at N-terminal c-Myc, expressed in E.coli.\n\nMolecular Weight: \n~83kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029535

Size

10ug

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris/HCl pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Purity

~51% (SDS-PAGE, Coomassie blue staining)

References

1. Fang S. et al., Mdm2 is a RING Finger-Dependent Ubiquitin Protein Ligase for Itself and p53. J Biol Chem., 275: 8945-8951, 2000. 2. Honda R. et al., Oncoprotein MDM2 is a Ubiquitin Ligase E3 for Tumor Suppressor p53. FEBS Lett. 420:25-27, 1997. 3. Marine J-C. and Lozano G. Mdm2-Mediated Ubiquitylation: p53 and Beyond. Cell Death and Differentiation, 17: 93-102, 2010. 4. Huart A-S. et al., CK1a Plays a Central Role in Mediating MDM2 Control of p53 and E2F-1 Protein Stability. J. Biol Chem., 284: 32384-32394, 2009. 5. Sigalas I. et al., Alternatively Spliced Mdm2 Transcripts with Loss of p53 Binding Domain Sequences: Transforming Ability and Frequent Detection in Human Cancer. Nat Med. 2: 912-917, 1996.