MDM4, ID (MDM4, MDMX, Protein Mdm4, Protein Mdmx, Double minute 4 protein, Mdm2-like p53-binding protein, p53-binding protein Mdm4) (Azide free) (HRP)

The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

038303-HRP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Xu, N., et al. Biochem. Biophys. Res. Commun. 401(3):417-421(2010)\nSarkari, F., et al. J. Mol. Biol. 402(5):825-837(2010)\nShimada, M., et al. Hum. Genet. 128(4):433-441(2010)\nBailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)\nFang, S., et al. PLoS ONE 5 (5), E10813 (2010) :

Additional Info

Human

Alternative Names

MDM4, MDMX, Protein Mdm4, Protein Mdmx, Double minute 4 protein, Mdm2-like p53-binding protein, p53-binding protein Mdm4