MDR2 (MRP2, Multi Drug Resistance Associated Protein, cMOAT, Canalicular Multi Organic Anion Transporter)

Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family. The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs. MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate. MRP2 is predominately expressed on the apical membranes in the liver and kidney proximal tubules. It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes.\nApplications:\nSuitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations.\nImmunohistochemistry (Frozen): 1:20 dilution on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse and a monoclonal mouse APAAP complex.\nImmunohistochemistry (Paraffin): Pretreatment with 0.01M citric acid, pH 6.0 in ddH2O at 100 degrees C for 5 min 3 times. After washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP).\nNote: Mouse samples are NOT suitable for use in IHC. \nWestern Blot: 1:20-1:50. Use an IgG (HRP) anti-mouse- as secondary antibody.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2825-17F

Size

125ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.7% BSA, 0.1% sodium azide

P Type

Mab

Purity

Supernatant

Isotype

IgG2b

References

1. Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science 271, 1126 (1996). 2. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res. 60, 5269 (2000) 3. Analysis of the in vivo functions of Mrp3: M.G. Belinsky, et al.; Mol. Pharmacol. 68, 160 (2005). 4. Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res. 57, 3537 (1997)

Additional Info

Recognizes an internal epitope of rat MRP2. Species Crossreactivity: human and mouse (Does not in react with mouse MRP2 in IHC). Does not cross-eact with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5.