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Melanoma-Associated Antigen

Cat no: M2869-04

Melanoma-Associated Antigen

Recognizes a melanoma-associated antigen present in the cytoplasm of melanoma cells and nevocellular nevi, in contrast to most Mabs against melanoma-associated antigens present in the membrane, which are not retained after formalin fixation. Therefore, the mab is very useful for paraffin sections in routine pathology. Crossreactivity with carcinoids, medullary carcinomas of the thyroid, and occasionally prostatic, primary breast, avarian, and lung carcinomas may be observed. No reaction is seen with basal cell carcinomas or brain tumors. \n\nApplications:\nSuitable for use in ELISA, Immunoblot and Immunohistochemistry (paraffin sections; no protease pretreatment required)\n\nRecommended Dilution: \nImmunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2869-04

Size

1ml

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

Additional Info

Reactive with human. Does not react with normal melanocytes or other normal tissues except for mastcells, histiocytes, salivary glands, bronchial glands, pancreatic und prostatic epithelium. In immunoblot the antibody reacts with diffuse protein bands between 25 and 100kD.

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