Membrin (GS27, Golgi SNARE of 27K)

Membrin, also known as GS27 (Golgi SNARE of 27K), is a 27kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (1). GS27 is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has shown that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other one contains syntaxin 5, GS27/membrin, rsec22 and rbet1 (2,3). These complexes are postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment (2,3).\n \nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 2ug/mL \nImmunoprecipitation: 2-4ug/mL \nImmunocytochemistry: 1:500 (4)\n\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4149-10

Size

25ug

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can, Ch/Bird, NHP, Prc, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Lowe, S.L., et al., Nature 389: 881-884 (1997). 2. Hay, J., et al., Cell 89: 149-158 (1997). 3. Hay, J., et al., J. Cell Biol. 141: 1489-1502 (1998). 4. Suvorova, E.S., et al., J. Biol. Chem. 276(25): 22,810-22,818 (2001).

Additional Info

This affinity purified antibody detects an ~27kD protein, corresponding to the apparent molecular mass of membrin, on SDS-PAGE immunoblots in samples from human, monkey, rat, canine, chicken, hamster, porcine and rabbit origins