Membrin (GS27, Golgi SNARE of 27kD)

Membrin, also known as GS27 (Golgi SNARE of 27 kD), is a 27 kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (2). Membrin is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has demonstrated that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other contains syntaxin 5, membrin, rsec22 and rbet1 (3,4). These complexes have been postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment (3,4).\n \nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 2ug/ml \nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nRat Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

M4149-20

Size

25ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in 25mM borate saline, 100mM boric acid, 75mMNaCl, 5mM EDTA (disodium salt), pH 8.4.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.. Lowe, S.L., Peter, F., Subramaniam, V.N., Wong, S.H. and Hong, W. (1997) Nature 389: 881-884. 2. Hay, J., Chao, D.S., Kuo, C.S. and Scheller, R.H. (1997) Cell 89: 149-158. 3. Hay, J., Klumperman, J., Oorschot, V., Steegmaier, M., Kuo, C.S. and Scheller, R.H. (1998) J. Cell Biol. 141: 1489-1502. \n\n

Additional Info

Recognizes rat Membrin at ~27kD. Species Crossreactivity: human, mouse, bovine, chicken, canine, Drosophila, hamster, monkey, porcine, rabbit and sheep. This band can be successfully competed in peptide inhibition studies on Western blots.