Description:
Mouse MSC growth medium was designed and tested for our Mouse Mesenchymal Stem cells (mMSC). This is the Mouse MSC growth medium supplement for M3005-34A. The Mouse MSC growth medium supplement includes fetal bovine serum and recombinant growth factors, and some lot-to-lot variability is unavoidable.
Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Use of Mouse MSC Growth Medium for Culturing Mouse MSC:
1. Thaw the supplement and add the entire volume (35ml) to the basal medium (500ml). Antibiotics may also be added if contamination is a concern.
2. Take the amount of MSC growth medium needed and warm to 37 degrees C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The Mouse MSC growth medium is stable for at least 1 month stored at 4 degrees C.
3. Refer to manual for MSC culture procedures using the Mouse MSC growth medium. We recommend changing medium every 2 days for regular cell culture.
Country of Origin:
Made in U.S.A.
Storage and Stability:
The MSC basal medium is stored at 4 degrees C. A change in color or appearance of precipitate may indicate deterioration. The Mouse MSC growth medium supplement is stored at -20 degrees C. If taking aliquots from the original bottle is necessary, dispense the contents and re-freeze in small aliquots to avoid repeated freezing-thawing cycles. Mouse MSC basal medium: Stable for 6 months from the date of receipt under proper storage condition. Mouse MSC growth medium supplement: Stable for at least 1 year from the date of receipt under proper storage condition.
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