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Metadherin (3D3, AEG1, LYRIC, MTDH, Astrocyte Elevated Gene Product-1)

Cat no: 145520

Metadherin (3D3, AEG1, LYRIC, MTDH, Astrocyte Elevated Gene Product-1)

MTDH (Metadherin; also LYRIC and Astrocyte Elevated Gene product-1/AEG1) is an 80kD membrane-bound protein that belongs to no known structural protein family. It has restricted expression, being found in breast ductal epithelium, striated muscle, select neurons and astrocytes, as well as multiple tumor types of diverse origin. MTDH is considered a mediator of Myc and Ha-Ras, and its activity is associated with stimulation of the beta-catenin/Wnt and NFkB signaling pathways. It is also believed to contribute to tight junction formation via an interaction with ZO-1. MTDH is also found in the nucleus where it complexes with CBP and p65. Human MTDH is 582 amino acids (aa) in length. It appears to be a type III transmembrane protein (N-terminus luminal/extracellular with no signal sequence) that is found embedded in both the plasma membrane and ER. It contains an N-terminal luminal region (aa1-48), plus a large 513aa cytoplasmic domain (aa70-582) that possesses at least 11 utilized Ser/Thr phosphorylation sites. There is the potential for ubiquitination, SUMOylation, acetylation and proteolytic processing, and this may account for multiple bands in SDS-PAGE that represent MWs of 20, 35, 50-55, 70-75, and 86kD. Over aa169-298, human MTDH shares 97% aa identity with mouse MDTH.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2ug/ml\nImmunocytochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145520

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.

P Type

Pab

Purity

Purified by Protein A or G affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Metadherin in direct ELISAs and Western blots. Species Crossreactivity: In direct ELISAs, 100% with recombinant mouse Metadherin.

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