Metal Transporter Protein, Mouse, Control Peptide (MTP1)

Control Peptide for M4695-50 (antiserum) and M4695-50B (affinity purified antibody).\n\nIron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A number of ferric reductases have been identified in yeast, plants and bacteria. The identity of such proteins remained unknown in the mammals. Most recently, a new gene called Dcytb (for duodenal cytochrome b) has been cloned and characterized in mouse duodenum that may function as mammalian ferric reductase. A novel iron-regulated gene that is homologous to the DMT1, termed Metal Transporter Protein (MTP1, human 571aa, mouse 570aa; ~90% similarity), has been identified. MTP1 is also described as iron-regulated transporter (IREG1) and Ferroportin1. MTP1 contains IRE in the 5'-UTR. It is expressed in tissues involved in body iron homeostasis including the developing and mature reticuloendothelial system, the duodenum and the pregnant uterus. MTP1 is also expressed in muscle and embryonic brain. In the adult mouse, MTP1 expression in the liver and duodenum are reciprocally regulated. Iron deficiency induces MTP1 expression in the duodenum and down-regulates expression in the liver. MTP1 may be involved in intracellular iron metabolism.\n\nApplications: \nSuitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml to coat ELISA plates.\nAntibody Blocking: 5-10ug per 1ug M4695-50B (affinity purified antibody) or per 1ul M4695-50 (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4695-50A

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Purity

Highly purified

References

1.

Additional Info

Synthetic peptide consisting of 19aa sequence located within the predicted cytoplasmic C-terminus of mouse MTP1. Does not distinguish betweein isoforms ferroprotein1/IREG1, ferroprotein 1 and IRG1/ferroprotein1. Species Sequence Homology: Mouse ferroprotein1/IREG1: 100%; rat ferroprotein 1: 90%; human IREG1/ferroprotein1: 78%.