Methionine Aminopeptidase 1D, Recombinant, Human (MetAP-1D, MAP1D)

Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is overexpressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1-43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).\n\nSource: Recombinant human Methionine Aminopeptidase 1D (rhMAP1D; residues 44-335; Accession # NP_954697) was expressed with a C-terminal 10X His tag in an insect cell line, Sf 21.\n\nDefinition: Measure by its ability to remove methionine from a fluorogenic peptide substrate Met-Gly-Pro-AMC in a coupled assay.\n\nSpecific Activity: (same/more than)30 pmol/min/ug.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Predicts 34kD and migrates as a 38kD band in SDS-PAGE under reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3015-99D

Size

20ug

Applications

WB

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris, 0.12M sodium chloride, 20% glycerol, pH 7.5.

Purity

(same/more than) 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin (same/less than) 1EU.

References

1. Leszczyniecka, M. et al., 2006, Oncogene 25:3471 - 3478.\n2. Lowther, W.T. and B.W. Matthews, 2000, Biochim. Biophys. Acta. 1477:157 - 167.\n3. Li, X. and Y.H. Chang, 1995, Proc. Natl. Acad. Sci. USA 92:12357 - 12361.\n4. Serero, A. et al., 2003, J. Biol. Chem. 278:52953 - 52963.