Methotrexate is a folic acid antagonist and is classified medically as an antineoplastic and antirheumatic agent.
Melting Point:
183-191C
Solubility:
Alkaline water, DMSO
Method for Determining Identity:
Proton NMR Spectroscopic Analysis
TLC Conditions:
SiO2; isopropanol; Water: NH4OH; 6:2:1; Visualized with UV and AMCS; Single spot; Rf=0.4
Water Content:
~12% by elemental analysis
Specific Rotation:
+18.6 (c=1, 0.1N NaOH)
Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.