mGluR2,3 (Metabotropic Glutamate Receptor)

Metabotropic glutamate receptors have been classified into three groups. In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission. Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC). Class II and class III mGluRs localized to the presynaptic membrane and are negatively coupled to adenylate cyclase. It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function. \n\nApplications:\nSuitable for use in Immunoblot, Immunoprecipitation, and Immunohistochemistry (paraffin embedded sections). Other applications have not been tested.\n\nRecommended Dilution: \nImmunoblot Analysis: 0.5-2ug/ml detects mGluR2/3 in 20ug of rat brain microsomal preparation. Note: Do not boil the microsomal preparation, heat to 37 degrees C for 15-30 minutes. 20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\nImmunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation.\nImmunohistochemistry: Detects mGluR 2/3 in paraffin embedded rat brain tissue sections.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3884-76

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Shigemoto, R., et al., J. Neuosci. 17: 7503

Additional Info

Recognizes mGluR2 and mGluR3, MW: 100-110kD. Species Crossreactivity: rat and mouse. Does not crossreact with rabbit. Species Sequence Homology: rat mGluR2: 100%; human and rat mGluR3: 89%.