Microphthalmia (Transcription Factor, MiTF)

Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kD and 56kD, while the longer Mi form runs as a cluster of bands at 60-70kD in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.1 \n\nMW of Antigen: 52-56kD doublet \n\nEpitope: N-terminal\n\nApplications and Recommended Dilutions: \nGel Supershift: Order Ab at 1mg/ml. See M3891-01X\nWestern Blotting (Not verified) \nImmunohistochemistry (Formalin/paraffin: 4-8ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. \n\nPositive Control: 501 Mel cells1. Melanoma \n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3891-01

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Can

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3891-01X

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Weilbaecher KN, et. al. J. Exp.Med. 1998, 187: 775

Additional Info

It does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.1 it reacts with both melanocytic and nonmelanocytic isoforms of Mi. Species Crossreactivity: human and canine. Does not react with mouse and rat.