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Minor Vault p193 Protein (Vault Poly ADP-Ribose Polymerase, VPARP, PARP4, ADP-Ribosyltransferase (NAD+ Poly (ADP-Ribose) Polymerase)-like 1, ADPRTL1, H5 Proline Rich, I-alpha-I Related, KIAA0177, OTTHUMP00000042288, PARP Related/I-alpha-I Related H5/Proli

Cat no: M3949-85F

Minor Vault p193 Protein (Vault Poly ADP-Ribose Polymerase, VPARP, PARP4, ADP-Ribosyltransferase (NAD+ Poly (ADP-Ribose) Polymerase)-like 1, ADPRTL1, H5 Proline Rich, I-alpha-I Related, KIAA0177, OTTHUMP00000042288, PARP Related/I-alpha-I Related H5/Proli

Applications:\nSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemstry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraformaldehyde (4%) fixed frozen): 1:10-1:20 Pretreat for 10 min with 20mM glycine in PBS, pH 7.5 and then for 10 min with 6N guanidine hydrochloride in 50mM Tris-HCl, pH 7.5.\nWestern Blot (ECL): 1:10-20\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M3949-85F

Size

1ml

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2b,k

References

1. Schroeijers AB et al., Cancer Res. 60: 1104, 2000; 2.. Kickhoefer VA et al., J. Cell Biol. 146: 917, 1999; 3. Kickhoefer VA et al., J. Bio. Chem. 273: 8971, 1998; 4. Scheper RJ et al., Cancer Res. 53: 1475, 1993; 5. Scheffer GL et al, Nature Med. 1: 578, 1995; 6. Izquierdo MA et al., Cytotechnology 27: 137, 1998.

Additional Info

Recognizes an internal epitope (aa491-494, HPGE) of the human minor vault p193 protein. Overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.

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