Monocarboxylate Transporter 4 (MCT4)

Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT4/MOT4/SLC16A3 (mouse 470-aa, rat 471-aa, human 465-aa; chromosome 17q25) is most closely related to MCT3, is prominently expressed in skeletal muscle and other cells with a high glycolytic rate such as tumor cells and white blood cells, suggesting an important role in lactic acid efflux. According to new nomenclature, the old MCT3 has now been reclassified as MCT4. This antibody has previously been listed as MCT31-S. Please consult the main MCT page for a detailed nomenclature.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-5ug/ml using ECL\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. (Catalog #M4470-29C)\nImmunohistochemistry: 5-10ug Paraformaldehyde fixed.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M4470-29B

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

US Biological application references: 1. Wang, Q. and Morris, M.E. (2007) Mol. Pharm. 35:1393-1399. 2. Wang, Q. et al., (2006) Mol. Pharm. 3:675-685. 1. Brauchi S 2004, Am J Physiol Cell Physiol, 288, 523-534, IHC 2. Wilson MC et al (1998) JBC 273, 15920-15926; 3. Price NT et al (1998) Biochem. J. 329, 321-328 (review); 4. Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).

Additional Info

Recognizes rat Monocarboxylate Transporter 4. No significant sequence homology exists with other MCTs.