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MORG1 (Mitogen-activated Protein Kinase Organizer 1, MAPK Organizer 1, WD Repeat Domain-containing Protein 83, WDR83)

Cat no: M4650-10

MORG1 (Mitogen-activated Protein Kinase Organizer 1, MAPK Organizer 1, WD Repeat Domain-containing Protein 83, WDR83)

MORG1 (mitogen-activated protein kinase organizer 1) is a member of the WD-40 protein family that was isolated as a binding partner of the extracellular signal-regulated kinase (ERK) pathway scaffold protein MP1. MORG1 specifically associates with several components of the ERK pathway, including MP1, Raf-1, MEK, and ERK, and stabilizes their assembly into an oligomeric complex. MORG1 facilitates ERK activation when cells are stimulated with lysophosphatidic acid, phorbol 12-myristate 13-acetate, or serum, but not in response to epidermal growth factor. Suppression of MORG1 by short interfering RNA leads to a marked reduction in ERK activity when cells are stimulated with serum. MORG1 is a component of a modular scaffold system that participates in the regulation of agonist-specific ERK signaling.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml \nImmunohistochemistry (Paraffin): 0.5-1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4650-10

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 0.2ml sterile dH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human MORG1. No crossreactivity with other proteins. Species Crossreactivity: mouse, rat.

Alternative Names

WD Repeat Domain 83

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