Mortality Factor 4 (MORF4, CSR, SEN, CSRB, SEN1)

Cellular senescence is the terminal non-dividing state that normal cells enter following completion of their proliferative potential. Fusions of immortal human cell lines with each other have led to their assignment to one of four complementation groups. Mortality factor 4 (MORF4) was identified as the lead member of a family of transcription factor-like proteins that reverses this immortal phenotype. Like other members in this family, MORF4 is localized to the nucleus and possesses transcription factor-like motifs such as helix-loop-helix and a leucine zipper motif that might allow it to form transcriptionally active homo- or heterodimers. MORF4 has been shown to bind to the transcription corepressors mSin3A and TLE, suggesting that together, these complexes may play a role in transcriptional repression of genes that lead to cellular senescence.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 Cell Lysate or Human Brain Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

146183

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Pereira-Smith OM and Smith JR. Genetic analysis of indefinite division in human cells: identification of four complementation groups. Proc. Natl. Acad. Sci. USA 1988; 85:6042-6. 2. Bertram MJ, Berube NG, Hang-Swanson X, et al. Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes. Mol. Cell. Biol. 1999; 1479-85. 3. Yochum GS and Ayer DE. Role for the mortality factors MORF4, MRGX, and MRG15 in transcriptional repression via associations with Pfl1, mSin3A, and transducin-like enhancer of split. Mol. Cell. Biol. 2002; 22:7868-76.

Additional Info

Recognizes human MORF4. Species Crossreactivity: mouse and rat.