BioAssay(TM) Aqueous Mounting Medium is an aqueous-based mounting medium designed for the permanent preservation of immunoperoxidase, alkaline phosphatase, and other histochemical stained tissues. Synthetic resins, such as BioAssay(TM) Organic Mounting Medium cannot be used with some immunostaining techniques because the organic solvent present in the media will dissolve alcohol-soluble chromogens such as AEC or Fast Red. An aqueous mounting media is thus needed for coverslipping.
Application:
Place drops of Bioassay(TM) Aqueous Mounting Medium over the tissue sample, and heat-dry. Hence, a liquid coverslip is formed. The hardened Mounting Medium has a superior refractive index when compared to other aqueous mounting media. Thus, slides are easier to read.
Note: Resolution for high magnification or photography can be improved by applying a permanent coverslip. The hardened Mounting Medium forms an imperious barrier to organic solvents and a permanent coverslip can be placed on the hardened polymer.
Protocol:
1. Stain slides with desired staining procedure.
2. Rinse slides in distilled water.
3. Blot-off excess water around tissue sections.
4. Place slides in horizontal position and apply BioAssay(TM) Aqueous Mounting Medium evenly over the tissue (Tissue must be completely covered).
5. Place slides in a drying oven set at 60 degrees C for 30 minutes or until slides are thoroughly dried. Slides are now ready for microscopic evaluation.
6. If a permanent coverslip is desired, dip slides in xylene and apply mounting medium (BioAssay(TM) Organic Mounting Medium cat# M4679) over the hardened Bioassay(TM) Aqueous Mounting Medium, and coverslip.
Note: Hardened Aqueous Mounting Medium can be removed from the slide by simply soaking the slide in warm water for approximately 10-30 minutes
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