Mouse alpha Macroglobulin ELISA kit is designed for detection of mouse alpha Macroglobulin in cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures alpha Macroglobulin in 4 hours. A polyclonal antibody specific for alpha Macroglobulin has been pre-coated onto a microplate. Alpha Macroglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for alpha Macroglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.