This Mouse Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total mouse IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. This assay does not distinguish IgG subclasses. The assay measures total mouse IgG in the 0.1-100ng/mL range. Samples giving mouse IgG levels above 100ng/mL should be diluted in BSA blocking buffer before use. A 1:1,000,000 to 1:20,000,000 dilution for serum is suggested for best results. The concentration of IgG in normal mouse serum ranges from 5 to 12 mg/mL. The kit does not cross react significantly with rat, human, guinea pig, or rabbit IgG.
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