Incubation periods = 120 minutes sample, 30 minutes conjugate and 10 minutes substrate. Each kit contains: sensitized 96-strip micro-well plate anti-Mouse KIM-1, 50 mL diluent, 50 mL concentrated wash solution, enzyme-antibody conjugate (HRP anti-Mouse KIM-1, 12mL TMB substrate, 12mL stop solution and a Mouse KIM-1 calibrator.