Mouse 13,14,Dihydro-15-keto-Prostaglandin F2alpha (PGFM) Enzyme Immunoassay kit measures PGFM present in non-invasively collected fecal and urine samples. The assay will also measure PGFM in plasma, serum and TCM samples. The kit has been developed for urine and extracted fecal samples and has been validated with Iberian lynx and other felid samples. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate and the reaction is read at 450nm wavelength.
PGFM has been suggested as a useful non-invasive pregnancy marker both in urine and fecal samples. It has been shown to be a precise, practical method for this application in these matrices for a number of species. Parallel courses were obtained when comparing urinary and fecal PGFM in a variety of felids and other species, and only a simple dilution of fecal extracts is necessary prior to analyses. Fecal PGFM analyses may allow pregnancy diagnosis in captive and free-ranging felids. Recent evidence has suggested that PGFM may also be a useful pregnancy marker in some other non-felid species.
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