In eukaryotic cells, the 3' end of mRNA is often extended with a poly(A) tail, a sequence of purine nucleotides. The poly(A) tail is a long chain of adenosine nucleotides of variable length. This tail is crucial for mRNA stability, transport, and translation. The analysis of mRNA's 3' poly(A) tail is a vital technique in studying the polyadenylation at mRNA molecules' 3' ends, with significant implications for gene regulation, cell development, and disease research.
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