MSI2 (RNA-binding Protein Musashi Homolog 2, Musashi-2, FLJ36569, MGC3245)

Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nEL4 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146847

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sakakibara S, Nakamura Y, Stoh H, et al. RNA-binding protein Musashi2: developmentally regulated expression in neuronal precursor cells and subpopulations of neurons in mammalian CNS. J. Neurosci. 2001; 21:8091-107 2. Kharas MG, Lengner CJ, Al-Shahrour F, et al. Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nat. Med. 2010; 16:903-8 3. Ito T, Kwon HY, Zimdahl B, et al. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature 2010; 466:765-8. 4. Griner LN and Reuther GW. Aggressive myeloid leukemia formation is directed by the Musashi2/Numb pathway. Cancer Biol. Ther. 2010; 10:979-82.

Additional Info

Recognizes human MSI2. Species Crossreactivity: mouse and rat.