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mTOR (Mammalian Target of Rapamycin, FK506 Binding Protein 12 Rapamycin Complex Associated Protein 1, FK506 Binding Protein12-rapamycin Associated Protein 1, FRAP1, FK506 Binding Protein12-rapamycin Associated Protein 2, FRAP2, FKBP12 Rapamycin Complex As

Cat no: M4696-02

mTOR (Mammalian Target of Rapamycin, FK506 Binding Protein 12 Rapamycin Complex Associated Protein 1, FK506 Binding Protein12-rapamycin Associated Protein 1, FRAP1, FK506 Binding Protein12-rapamycin Associated Protein 2, FRAP2, FKBP12 Rapamycin Complex As

FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235kD protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4696-02

Size

100ul

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Tris-citrate/phsophate [pH7-8]

P Type

Pab

Purity

Serum

Additional Info

Specific for human Mammalian Target of Rapamycin (mTOR). Species Crossreactivity: rat. Other species have not been tested.

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