Mva1269I (BsmI)

5'-G A A T G C N?-3' \n3'-C T T A C?G N-5'\n\nUnit Definition: \nOne unit is defined as the amount of Mva1269I required to digest 1ug of lambda DNA in 1 hour at 37 degrees C in 50ul of assay buffer.\n\nIncubation Temperature: \n37 degrees C\n\nDilution Buffer: \n10mM Tris-HCl (pH 7.5 at 25 degrees C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.\n\nSupplied with:\nR1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid containing 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.\nR1625-04: Restriction Enzyme Buffer E, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM magnesium chloride, 100mM potassium chloride, 0.1mg/ml BSA.\n\nThermal Inactivation: \nMva1269I is inactivated by incubation at 65 degrees C for 20min. \n\nEnzyme Properties:\nMethylation Effects:\nDam, Dcm, EcoKI: Never overlaps - no effect\nCpG: May overlap - no effect\nEcoBI: May overlap - effect not determined\n\nStability during Prolonged Incubation: A minimum of 0.1units of Mva1269I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degrees C. \n\nDigestion of Agarose-embedded DNA: A minimum of 5 units of Mva1269I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.\n\nNumber of Recognition Sites in DNA: \nLambda: 46\nPhiX174: 4\npBR322, pUC57, M13mp18/19: 1\npUC18/19, pTZ19R/U: 0\n\nThermal Inactivation: \nMva1269I is inactivated by incubation at 65 degrees C for 20min. \n\nEnzyme Properties:\nMethylation Effects:\nDam, Dcm, EcoKI: Never overlaps - no effect\nCpG: May overlap - no effect\nEcoBI: May overlap - effect not determined\n\nStability during Prolonged Incubation: A minimum of 0.1units of Mva1269I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degrees C. \n\nDigestion of Agarose-embedded DNA: A minimum of 5 units of Mva1269I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.\n\nNumber of Recognition Sites in DNA: \nLambda: 46\nPhiX174: 4\npBR322, pUC57, M13mp18/19: 1\npUC18/19, pTZ19R/U: 0

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